Emerging in the late Ediacaran Period, Shaanxilithes witnessed the biological evolution and ecological environment changes during the Ediacaran-Cambrian transitional interval, which has a significant role in stratigraphic correlation. This paper pre-sents the first report of the Shaanxilithes ningqiangensis Xing, Yue and Zhang, 1984 preserved in argillaceous limestone in Jiucheng Member of Zhujiaqing Section in Huize, Yunnan. The specimens show ribbons with jagged edges and annulations, as well as the distribution of discoidal components. When compared to the material of Shaanxilithes from Wangjiawan Sec-tion in Jinning, the newly discovered specimens from Zhujiaqing type Section are relatively thinner, longer and more frag-mented. According to the results of Micro X-ray Fluorescence Spectrometer (μ-XRF), the new carbonate-hosted specimens show a high concentration of silicon, aluminum and potassium, while the morphology of the material from Wangjiawan Sec-tion are prominent in iron mapping; the concentration of calcium in the fossil and matrix is much higher in the new material; sulphur only shows a little concentration in the new material, by contrast no signal indicated in the latter. These new evidence indicates that the problematic Shaanxilithes can be preserved not only in clastic settings, but also in an argillaceous limestone environment. In addition, it may suggest that the materials of Shaanxilithes were fossilized by illite, and the degree of weath-ering in different lithofacies results in different preservation of fossils recovered from two sections. Shaanxilithes has been reported in the late Ediacaran in East Yunnan, northwest margin of India, Namibia and southwest margin of North China Platform. The new discovery of Shaanxilithes in the Huize region of Eastern Yunnan further demonstrates the worldwide distribution of this taxon, which could be used as an index fossil in the upper Ediacaran successions for global stratigraphic correlation. Based on the morphological observations and the results of elemental mappings and semi-quantitative elemental analyses, this paper discussed their morphological and taphonomic information and phylogenetic affinities, which provided new perspectives on exploring the biological and ecological evolution of metazoans during the Ediacaran-Cambrian transi-tional interval.