Amber, as an organic gem, is fossilized natural resin widely distributed around the world, especially Baltic region in Europe, Dominica-Mexico in Central America, and Myanmar in Asia. Insects are the most common inclusions in amber, while verte-brate inclusions are the rarest. However, compared to vertebrate fossils from sedimentary rocks, vertebrate inclusions trapped in amber pieces can provide additional information about the soft tissues, primitive death states, and living environment, as well as more visualized and refined 3D morphological information, all of which are important for studies in evolution, palae-oenvironmental restoration, palaeoecology and palaeoethology, which is more intuitive, stereoscopic and detailed than bone fossils. This paper reviews vertebrate inclusions from various amber biotae, including non-avian dinosaurs, aves, mammals, reptiles and amphibians, as well as their evolutionary implication, and also provides a framework of future researches.