The Zhepure Formation of Gamba stands for the youngest marine sediments in southern Xizang(Tibet). Nine samples containing ostrocods were collected for this study from the upper sand-shale member of the Zhepure Formatiom in Zongpuxi section, Gamba county of southern Tibet. Two Ostracod assemblages are recognized (in ascending order): 1. Paracypris mayaensis sp. nov.-Bairdia zongpuxiensis sp. nov. assemblage, 2. Phlyctenophora zongpuensis-Semicytherura subsymmetros assemblage. The second assemblage is dated as late Lutetian to early Priabonian of Eocene. It indicates that the sea water did not withdraw from the southern Tibet until early Priabonian. Six species, including 2 new species, have been described and illustrated in this paper.