在MRC1000型激光扫描共聚焦显微镜下,观察具有自发荧光的孢子、花粉、沟鞭藻以及疑源类等不同时代的化石标本,发现现代和第四纪孢粉具有较强的自发荧光,古生代的孢子自发荧光强度最弱。后者很难聚焦成清晰的二维投影图像。在观察孢粉样品过程中,选择合适的激光波长及激光扫描强度是关键的技术问题。一般以氪、氩离子激发为效果最佳,以波长488,568,647nm最合适。

A preliminary observation of some spore-pollen fossils has been made using Laser Scanning Confocal Microscope (LSCM). Some quite new features about the texture and ultrastructure of pollen have been detected for the first time, which opens up a new application of this advanced technique to microfossil study. After the successful observation of fossil pollen, the LSCM in the application of fossil study can be outlined as follows:1) The most pollen and autofluorescene fossils shine blue (488nm), yellow (568nm) or red lights (647nm) under the fluorescence microscope. According to the Fluorophore excitation/emission maxima spectrum, the argon ion will be the most effective one to excite the fossil samples. 2) To observe the internal structure of fossil pollen without destroying the specimens themselves. The LSCM could make a series of virtually optical sections with higher resolution than conventional light microscope. 3) To reconstruct the 3-D image after sequencing continuous optical sections to the fossils, which allows the researcher to view much detailed 3-D stretch and changes of certain structure in both internal and external views.

Q913.84 TH742.64

国家重点实验室基金